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KMID : 0043320030260121036
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Archives of Pharmacal Research
2003 Volume.26 No. 12 p.1036 ~ p.1041
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Characteristics of Chitosanases from Aspergillus fumigatus KB-1
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Eom Tae-Kyoung
Lee Kang-Man
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Abstract
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Two chitosanases produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chitosanase I) and 23.38 kDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined as follows: YNLPNNLKQIYDKHKGKXSXVLAKGFTN. The optimum pH of the chitosanase I and II was 6.5 and 5.5, respectively. The optimum temperatures were for chitosanase land for chitosanase II. Hydrolysis products of two chitosanases were analyzed by HPLC and GPC. Chitosanase I hydrolyzed substrate to glucosamine. Chitosanase II produced chitooligosaccharides.
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KEYWORD
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Chitosan, Chitosanase, Aspergillus fumigatus KB-1, N-terminal amino acid sequence
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